Suppression of Notch Signaling in Osteoclasts Improves Bone Regeneration and Healing.

ABSTRACT: Owing to the central role of osteoclasts in bone physiology and remodeling, manipulation of their maturation process provides a potential therapeutic strategy for treating bone diseases. To investigate this, we genetically inhibited the Notch signaling pathway in the myeloid lineage, which includes osteoclast precursors, using a dominant negative form of MAML (dnMAML) that inhibits the transcriptional complex required for downstream Notch signaling. Osteoclasts derived from dnMAML mice showed no significant differences in early osteoclastic gene expression compared to the wild type. Further, these demonstrated significantly lowered resorption activity using bone surfaces while retaining their osteoblast stimulating ability using ex vivo techniques. Using in vivo approaches, we detected sig

SUBMITTER: Goel PN 

PROVIDER: S-EPMC6739141 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature