Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma.

ABSTRACT: Endothelial-to-mesenchyme-like transition (Endo-MT) of trabecular meshwork (TM) cells is known to be associated with primary open angle glaucoma (POAG). Here, we investigated whether the prion protein (PrP^C), a neuronal protein known to modulate epithelial-to-mesenchymal transition in a variety of cell types, is expressed in the TM, and plays a similar role at this site. Using a combination of primary human TM cells and human, bovine, and PrP-knock-out (PrP^-/-) mouse models, we demonstrate that PrP^C is expressed in the TM of all three species, including endothelial cells lining the Schlemm's canal. Silencing of PrP^C in primary human TM cells induces aggregation of β1-integrin and upregulation of α-smooth muscle actin, fibronectin, collagen 1A, vi

SUBMITTER: Ashok A 

PROVIDER: S-EPMC6739364 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature