Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition.

ABSTRACT: Synthetic biology and metabolic engineering have expanded the possibilities for engineered cell-based systems. The addition of non-native biosynthetic and regulatory components can, however, overburden the reprogrammed cells. In order to avoid metabolic overload, an emerging area of focus is on engineering consortia, wherein cell subpopulations work together to carry out a desired function. This strategy requires regulation of the cell populations. Here, we design a synthetic co-culture controller consisting of cell-based signal translator and growth-controller modules that, when implemented, provide for autonomous regulation of the consortia composition. The system co-opts the orthogonal autoinducer AI-1 and AI-2 cell-cell signaling mechanisms of bacterial quorum sensing (QS) to enable cr

SUBMITTER: Stephens K 

PROVIDER: S-EPMC6739400 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature