Mechanisms underlying TNFα-induced enhancement of force generation in airway smooth muscle.

ABSTRACT: Airway diseases such as asthma are triggered by inflammation and mediated by proinflammatory cytokines such as tumor necrosis factor alpha (TNFα). Our goal was to systematically examine the potential mechanisms underlying the effect of TNFα on airway smooth muscle (ASM) contractility. Porcine ASM strips were incubated for 24 h with and without TNFα. Exposure to TNFα increased maximum ASM force in response to acetylcholine (Ach), with an increase in ACh sensitivity (hyperreactivity), as reflected by a leftward shift in the dose-response curve (EC₅₀ ). At the EC₅₀ , the [Ca²⁺ ]_cyt response to ACh was similar between TNFα and control ASM, while force increased; thus, Ca²⁺ sensitivity appeared to increase. Exposure to TNFα increased the ba

SUBMITTER: Sieck GC 

PROVIDER: S-EPMC6739507 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature