Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over-looked physiological factors.

ABSTRACT: To bridge biological and biochemical disciplines, the relationship between in vitro protein biochemical function and in vivo activity must be established. Such studies can (a) help determine whether properties measured in simple, dilute solutions extrapolate to the complex in vivo conditions and (b) illuminate cryptic biological factors that are new avenues for study. We have explored the in vivo-in vitro relationship for chimeras built from LacI/GalR transcription regulators. In prior studies of individual chimeras, amino acid changes that altered in vitro DNA binding affinity exhibited correlated changes in in vivo transcription repression. However, discrepancies arose when the two datasets were compared to each other: Although their DNA binding domains were identical and their in vitro

SUBMITTER: Tungtur S 

PROVIDER: S-EPMC6739814 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature