Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.

ABSTRACT:

Background

Oligosaccharides of glycoprotein, particularly negatively-charged sialylated N-glycans, on the surface of lymphomas play important roles in cell-cell interactions and bind immunoglobulin-like lectins, causing inflammatory responses and bioregulation. However, their characterizations have largely been unknown in central nervous system (CNS) lymphoma.

Methods

Here, we investigated expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens.

Results

We first generated methotrexate (MTX)-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma and determined N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK,

SUBMITTER: Takashima Y 

PROVIDER: S-EPMC6739943 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature