Claudin-12 is not required for blood-brain barrier tight junction function.

ABSTRACT:

Background

The blood-brain barrier (BBB) ensures central nervous system (CNS) homeostasis by strictly controlling the passage of molecules and solutes from the bloodstream into the CNS. Complex and continuous tight junctions (TJs) between brain endothelial cells block uncontrolled paracellular diffusion of molecules across the BBB, with claudin-5 being its dominant TJs protein. However, claudin-5 deficient mice still display ultrastructurally normal TJs, suggesting the contribution of other claudins or tight-junction associated proteins in establishing BBB junctional complexes. Expression of claudin-12 at the BBB has been reported, however the exact function and subcellular localization of this atypical claudin remains unknown.

Methods

We created claudin-12-lacZ-knock-in C57

SUBMITTER: Castro Dias M 

PROVIDER: S-EPMC6739961 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature