The first enhancer in an enhancer chain safeguards subsequent enhancer-promoter contacts from a distance.

ABSTRACT:

Background

Robustness and evolutionary stability of gene expression in the human genome are established by an array of redundant enhancers.

Results

Using Hi-C data in multiple cell lines, we report a comprehensive map of promoters and active enhancers connected by chromatin contacts, spanning 9000 enhancer chains in 4 human cell lines associated with 2600 human genes. We find that the first enhancer in a chain that directly contacts the target promoter is commonly located at a greater genomic distance from the promoter than the second enhancer in a chain, 96 kb vs. 45 kb, respectively. The first enhancer also features higher similarity to the promoter in terms of tissue specificity and higher enrichment of loop factors, suggestive of a stable primary contact with the promote

SUBMITTER: Song W 

PROVIDER: S-EPMC6739990 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature