Kinetics-Based Structural Requirements of Human Immunoglobulin G Binding Peptides.

ABSTRACT: Currently, antibodies are widely used not only in research but also in therapy. Hence, peptides that selectively bind to the fragment crystallizable site of an antibody have been extensively utilized in various research efforts such as the preparation of antibody-drug conjugates (ADC). Consequently, appropriate peptides that bind to immunoglobulin G (IgG) with a specific K _d value and also k _on and k _off values will be useful in different applications, and these kinetic parameters have been perhaps overlooked but are key to development of peptide ligands with advantageous binding properties. We prepared structural derivatives of IgG-binding peptide 1 and evaluated the binding affinity and kinetic rates of the products by surface

SUBMITTER: Muguruma K 

PROVIDER: S-EPMC6740044 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature