The distribution of calcium buffering proteins in the turtle cochlea.

ABSTRACT: Hair cells of the inner ear contain high concentrations of calcium-binding proteins that limit calcium signals and prevent cross talk between different signaling pathways during auditory transduction. Using light microscope immunofluorescence and post-embedding immunogold labeling in the electron microscope, we characterized the distribution of three calcium-buffering proteins in the turtle cochlea. Both calbindin-D28k and parvalbumin-beta were confined to hair cells in which they showed a similar distribution, whereas calretinin was present mainly in hair-cell nuclei but also occurred in supporting cells and nerve fibers. The hair-cell concentration of calbindin-D28k but not of parvalbumin-beta increased from the low- to high-frequency end of the cochlea. Calibration against standards con

SUBMITTER: Hackney CM 

PROVIDER: S-EPMC6740801 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature