Studies of NMDA receptor function and stoichiometry with truncated and tandem subunits.

ABSTRACT: The subunits that compose eukaryotic glutamate ion channel receptors have three transmembrane domains (TMs) and terminate with intracellular tails that are important for controlling channel expression and localization. Truncation of NMDA receptor subunits before the final TM showed that this TM and intracellular tail region are necessary to form functional channels. However, it is shown here that these truncated subunits may be partially rescued by coexpressing the final TM and tail as a separate protein. The whole-cell currents so produced are somewhat lower than with full-length subunits, and they do not show the sag characteristic of currents from channels containing NR1 and NR2A subunits in the continued presence of an agonist. In addition, these truncated subunits were joined to full-

SUBMITTER: Schorge S 

PROVIDER: S-EPMC6742241 | biostudies-literature | 2003 Feb

REPOSITORIES: biostudies-literature