Packaging functionally important plasma proteins into the α-granules of human-induced pluripotent stem cell-derived megakaryocytes.

ABSTRACT: The contents of platelet α-granules arrive via a number of pathways; some are synthesized by megakaryocytes (MKs), for example, von Willebrand factor (VWF), whereas others are endocytosed from plasma, for example, fibrinogen (Fgn) and factor V (FV). Currently, almost all in vitro-induced pluripotent stem cell (iPSC)-derived MKs are generated under serum-free conditions, and their α-granule cargoes lack components that would normally be taken up from plasma during the course of megakaryopoiesis. How this might affect the ability of in vitro-derived platelets to contribute fully to haemostasis is not known. The purpose of this investigation was to examine whether "feeding" human plasma to iPSC-derived MKs might result in loading their α-granules with physiologically important proteins. iPSCs

SUBMITTER: Zhang N 

PROVIDER: S-EPMC6742440 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature