Identification and analysis of RNA editing sites in chloroplast transcripts of kenaf (Hibiscus cannabinus L.).

ABSTRACT: RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predicted and identified using bioinformatics and RT-PCR analysis. The prediction results showed a total of 48 editing sites distributed in 22 genes, all of them were C to U conversion leading to amino acid changes. Further analysis of the position of RNA editing sites revealed that except 11 editing sites located at the first codon base, the other editing sites were found at the second codon base. Then four genes were randomly selected to validate the editing sites. Results showed t

SUBMITTER: Tang D 

PROVIDER: S-EPMC6742680 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature