Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome.

ABSTRACT: The biotechnological process of biogas production from organic material is carried out by a diverse microbial community under anaerobic conditions. However, the complex and sensitive microbial network present in anaerobic degradation of organic material can be disturbed by increased ammonia concentration introduced into the system by protein-rich substrates and imbalanced feeding. Here, we report on a simulated increase of ammonia concentration in a fed batch lab-scale biogas reactor experiment. Two treatment conditions were used simulating total ammonia nitrogen concentrations of 4.9 and 8.0 g/L with four replicate reactors. Each reactor was monitored concerning methane generation and microbial composition using 16S rRNA gene amplicon sequencing, while the transcriptional activity of the

SUBMITTER: Fischer MA 

PROVIDER: S-EPMC6742706 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature