Rhoptry and Dense Granule Secreted Effectors Regulate CD8⁺ T Cell Recognition of Toxoplasma gondii Infected Host Cells.

ABSTRACT: Toxoplasma gondii secretes rhoptry (ROP) and dense granule (GRA) effector proteins to evade host immune clearance mediated by interferon gamma (IFN-γ), immunity-related GTPase (IRG) effectors, and CD8⁺ T cells. Here, we investigated the role of parasite-secreted effectors in regulating host access to parasitophorous vacuole (PV) localized parasite antigens and their presentation to CD8⁺ T cells by the major histocompatibility class I (MHC-I) pathway. Antigen presentation of PV localized parasite antigens by MHC-I was significantly increased in macrophages and/or dendritic cells infected with mutant parasites that lacked expression of secreted GRA (GRA2, GRA3, GRA4, GRA5, GRA7, GRA12) or ROP (ROP5, ROP18) effectors. The ability of various secreted GRA or ROP eff

SUBMITTER: Rommereim LM 

PROVIDER: S-EPMC6742963 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature