PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations.

ABSTRACT: In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the method is simple and makes use of a commercial kit, with additional step of concentrating the captured library by ethanol precipitation. The accuracy of the PCR-free method was found to be equivalent to that of unique molecular identifier-corrected analysis method, which is the commonly used method to detect rare mutations. Thus, the PCR-free whole exome sequencing method is cost-effective as well as efficient in detecting rare mutations.

SUBMITTER: Yamaguchi I 

PROVIDER: S-EPMC6743761 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature