High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach.

ABSTRACT:

Background

Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.

Methods

In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.

Results

Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papill

SUBMITTER: Mollerup S 

PROVIDER: S-EPMC6743825 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature