[Retinal transcriptome profile in mice following dexamethasone treatment for endotoxin-induced uveitis].

ABSTRACT:

Objective

To investigate the changes in retinal transcriptome profile of mice with endotoxin-induced uveitis (EIU)following dexamethasone (DEX) treatment and explore the mechanisms underlying the therapeutic effect of DEX.

Methods

EIU was induced in BALB/c mice by intravitreal injection of 125 ng lipopolysaccharide (LPS), followed by topical applicationof DEX (0.1%) eye drops every 4 h for 24 h. The anterior chamber inflammation was examined with a slit lamp and the clinicalscores were assessed. The morphological changes in the eyes were assessed at 24 h after LPS injection. The retinas wereharvested for analysis of transcriptome profile using the next-generation sequencing (NGS)-based RNA sequencing (RNA-seq), and the expressions of the inflammatory cytokines and the differ

SUBMITTER: Peng YU 

PROVIDER: S-EPMC6744048 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature