[Screening and verification of key genes in T-cell acute lymphoblastic leukemia].

ABSTRACT:

Objective

To explore the key genes in T-cell acute lymphoblastic leukemia (T-ALL) using bioinformatics method to better understand the pathogenic mechanisms of T-ALL.

Methods

The gene expression profiles of GSE14317 were obtained from Gene Expression Omnibus database. The differentially expressed genes (DEGs) in T-ALL were analyzed using R package Limma. The online analysis tool DAVID was used to perform the functional and pathway enrichment analysis. The protein-protein interaction network was constructed by STRING and visualized by Cytoscape. Based on the JASPAR database, the transcription factors (TFs) of the hub genes were obtained. RT-PCR was used to test the mRNA expression level of the key genes.

Results

A total of 1443 DEGs were identified, including 800 up-re

SUBMITTER: Jiang GJ 

PROVIDER: S-EPMC6744169 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature