Evaluating the predictions of the protein stability change upon single amino acid substitutions for the FXN CAGI5 challenge.

ABSTRACT: Frataxin (FXN) is a highly conserved protein found in prokaryotes and eukaryotes that is required for efficient regulation of cellular iron homeostasis. Experimental evidence associates amino acid substitutions of the FXN to Friedreich Ataxia, a neurodegenerative disorder. Recently, new thermodynamic experiments have been performed to study the impact of somatic variations identified in cancer tissues on protein stability. The Critical Assessment of Genome Interpretation (CAGI) data provider at the University of Rome measured the unfolding free energy of a set of variants (FXN challenge data set) with far-UV circular dichroism and intrinsic fluorescence spectra. These values have been used to calculate the change in unfolding free energy between the variant and wild-type proteins at zero c

SUBMITTER: Savojardo C 

PROVIDER: S-EPMC6744327 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature