Efficient genome-wide first-generation phenotypic screening system in mice using the piggyBac transposon.

ABSTRACT: Genome-wide phenotypic screens provide an unbiased way to identify genes involved in particular biological traits, and have been widely used in lower model organisms. However, cost and time have limited the utility of such screens to address biological and disease questions in mammals. Here we report a highly efficient piggyBac (PB) transposon-based first-generation (F1) dominant screening system in mice that enables an individual investigator to conduct a genome-wide phenotypic screen within a year with fewer than 300 cages. The PB screening system uses visually trackable transposons to induce both gain- and loss-of-function mutations and generates genome-wide distributed new insertions in more than 55% of F1 progeny. Using this system, we successfully conducted a pil

SUBMITTER: Chang H 

PROVIDER: S-EPMC6744845 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature