Generation of Zika virus-specific T cells from seropositive and virus-naive donors for potential use as an autologous or "off-the-shelf" immunotherapeutic.

ABSTRACT:

Background

Zika virus (ZIKV) infection can cause severe birth defects in newborns with no effective currently available treatment. Adoptive transfer of virus-specific T cells has proven to be safe and effective for the prevention or treatment of many viral infections, and could represent a novel treatment approach for patients with ZIKV infection. However, extending this strategy to the ZIKV setting has been hampered by limited data on immunogenic T-cell antigens within ZIKV. Hence, we have generated ZIKV-specific T cells and characterized the cellular immune responses against ZIKV antigens.

Methods

T-cell products were generated from peripheral blood of ZIKV-exposed donors, ZIKV-naive adult donors and umbilical cord blood by stimulation with pentadecamer (15mer) overlapping

SUBMITTER: Hanajiri R 

PROVIDER: S-EPMC6744970 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature