Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities.

ABSTRACT:

Background

Non-alcoholic steatohepatitis (NASH) is a common disease and feeding mice a methionine-choline-deficient (MCD) diet is a frequently used model to study its pathophysiology. Genetic and environmental factors influence NASH development and liver lipid content, which was studied herein using C57BL/6 J mice bred in two different animal facilities.

Methods

Age-matched male C57BL/6 J mice bred in two different animal facilities (later on referred to as WT1 and WT2) at the University Hospital of Regensburg were fed identical MCD or control chows for 2 weeks. Hepatic gene and protein expression and lipid composition were determined.

Results

NASH was associated with increased hepatic triglycerides, which were actually higher in WT1 than WT2 liver in both dietary gro

SUBMITTER: Rein-Fischboeck L 

PROVIDER: S-EPMC6745065 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature