The association between TNP2 gene polymorphisms and Iranian infertile men with varicocele: A case-control study.

ABSTRACT:

Background

Numerous researches have provided great evidence that revealed the relationship between varicocele and sperm DNA damage.

Objective

Because of the crucial role of nuclear transition proteins (TPs) in sperm DNA condensation and integrity, this case-control study was designed to study TNP2 gene nucleotide variations in Iranian patients with varicocele.

Materials and methods

PCR-SSCP and DNA sequencing were used to search for mutations in exons 1 & 2 of the TNP2 gene in 156 infertile patients with varicocele and 150 fertile men.

Results

The results of sequencing showed three variants at positions c.301C░>░T (p.R101C), c.391C░>░T (p.R131░W), and g.IVS1-26G░>░C (rs8043625) of TNP2 gene. It was found that varicocele risk in men who have

SUBMITTER: Heidari MM 

PROVIDER: S-EPMC6745086 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature