Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors.

ABSTRACT: Chemically induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. Three-dimensional (3D) structures generated from liver progenitor cells possess wide applications in cell transplantation, disease model, and drug testing. Here, we report on the spontaneous formation of 3D cystic structures comprising maturing rat CLiPs on gelatin-coated dishes. Our 3D cysts contained Alb^+/+CK19^+/- and Ck19^+/+Alb^+/- cells. These cell types gradually diverged into specialized mature cells, as demonstrated by the expression of mature biliary markers (Cftr, Ae2, and Aqp1) and hepatic markers (Alb and Mrp2). The 3D cysts also expressed functional multidrug resistance protein 1 (Mdr1), as indicated by epithelial efflux of rhodamin

SUBMITTER: Huang Y 

PROVIDER: S-EPMC6745155 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature