Expression of the Mxra8 Receptor Promotes Alphavirus Infection and Pathogenesis in Mice and Drosophila.

ABSTRACT: Mxra8 is a recently described receptor for multiple alphaviruses, including Chikungunya (CHIKV), Mayaro (MAYV), Ross River (RRV), and O'nyong nyong (ONNV) viruses. To determine its role in pathogenesis, we generated mice with mutant Mxra8 alleles: an 8-nucleotide deletion that produces a truncated, soluble form (Mxra8^Δ8/Δ8) and a 97-nucleotide deletion that abolishes Mxra8 expression (Mxra8^Δ97/Δ97). Mxra8^Δ8/Δ8 and Mxra8^Δ97/Δ97 fibroblasts show reduced CHIKV infection in culture, and Mxra8^Δ8/Δ8 and Mxra8^Δ97/Δ97 mice have decreased infection of musculoskeletal tissues with CHIKV, MAYV, RRV, or ONNV. Less foot swelling is observed in CHIKV-infected Mxra8 mutant mice, which correlated with fewer infiltrating neutrophils and cyto

SUBMITTER: Zhang R 

PROVIDER: S-EPMC6745702 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature