Transcriptomic analysis of differential gene expression during chick periocular neural crest differentiation into corneal cells.

ABSTRACT:

Background

Multipotent neural crest cells (NCC) contribute to the corneal endothelium and keratocytes during ocular development, but the molecular mechanisms that underlie this process remain poorly understood. We performed RNA-Seq analysis on periocular neural crest (pNC), corneal endothelium, and keratocytes and validated expression of candidate genes by in situ hybridization.

Results

RNA-Seq profiling revealed enrichment of genes between pNC and neural crest-derived corneal cells, which correspond to pathways involved in focal adhesion, ECM-receptor interaction, cell adhesion, melanogenesis, and MAPK signaling. Comparisons of candidate NCC genes to ocular gene expression revealed that majority of the NCC genes are expressed in the pNC, but they are either differentially e

SUBMITTER: Bi L 

PROVIDER: S-EPMC6746171 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature