Recapitulating endocrine cell clustering in culture promotes maturation of human stem-cell-derived β cells.

ABSTRACT: Despite advances in the differentiation of insulin-producing cells from human embryonic stem cells, the generation of mature functional β cells in vitro has remained elusive. To accomplish this goal, we have developed cell culture conditions to closely mimic events occurring during pancreatic islet organogenesis and β cell maturation. In particular, we have focused on recapitulating endocrine cell clustering by isolating and reaggregating immature β-like cells to form islet-sized enriched β-clusters (eBCs). eBCs display physiological properties analogous to primary human β cells, including robust dynamic insulin secretion, increased calcium signalling in response to secretagogues, and improved mitochondrial energization. Notably, endocrine cell clustering induces metabolic maturation by dr

SUBMITTER: Nair GG 

PROVIDER: S-EPMC6746427 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature