Calcium dysregulation mediates mitochondrial and neurite outgrowth abnormalities in SOD2 deficient embryonic cerebral cortical neurons.

ABSTRACT: Mitochondrial superoxide dismutase 2 (SOD2) is a major antioxidant defense enzyme. Here we provide evidence that SOD2 plays critical roles in maintaining calcium homeostasis in newly generated embryonic cerebral cortical neurons, which is essential for normal mitochondrial function and subcellular distribution, and neurite outgrowth. Primary cortical neurons in cultures established from embryonic day 15 SOD2^+/+ and SOD2^-/- mice appear similar during the first 24 h in culture. During the ensuing two days in culture, SOD2^-/- neurons exhibit a profound reduction of neurite outgrowth and their mitochondria become fragmented and accumulate in the cell body. The structural abnormalities of the mitochondria are associated with reduced levels of phosphorylated (S63

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC6748124 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature