Novel fluorescence techniques to quantitate renal cell biology.

ABSTRACT: Fluorescence microscopy techniques are powerful tools to study tissue dynamics, cellular function and biology both in vivo and in vitro. These tools allow for functional assessment and quantification along with qualitative analysis, thus providing a comprehensive understanding of various cellular processes under normal physiological and disease conditions. The main focus of this chapter is the recently developed method of serial intravital multiphoton microscopy that has helped shed light on the dynamic alterations of the spatial distribution and fate of single renal cells or cell populations and their migration patterns in the same tissue region over several days in response to various stimuli within the living kidney. This technique is very useful for studying in vivo the molecular and c

SUBMITTER: Shroff UN 

PROVIDER: S-EPMC6748388 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature