Compositional changes to the ileal microbiome precede the onset of spontaneous ileitis in SHIP deficient mice.

ABSTRACT: Inflammatory bowel disease, encompassing both ulcerative colitis and Crohn's disease, is characterized by chronic, relapsing-remitting gastrointestinal inflammation of unknown etiology. SHIP deficient mice develop fully penetrant, spontaneous ileitis at 6 weeks of age, and thus offer a tractable model of Crohn's disease-like inflammation. Since disruptions to the microbiome are implicated in the pathogenesis of Crohn's disease, we conducted a 16S rRNA gene survey of the ileum, cecum, colon, and stool contents of SHIP^+/+ and SHIP^-/- mice. We predicted that diversity and compositional changes would occur after, and possibly prior to, the onset of overt disease. No differences were found in alpha diversity, but significant changes in beta diversity and specific commensal

SUBMITTER: Dobranowski PA 

PROVIDER: S-EPMC6748580 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature