Proteomics and phosphoproteomics study of LCMT1 overexpression and oxidative stress: overexpression of LCMT1 arrests H₂O₂-induced lose of cells viability.

ABSTRACT:

Objectives

Protein phosphatase 2A (PP2A), a major serine/threonine phosphatase, is also known to be a target of ROS. The methylation of PP2A can be catalyzed by leucine carboxyl methyltransferase-1 (LCMT1), which regulates PP2A activity and substrate specificity.

Methods

In the previous study, we have showed that LCMT1-dependent PP2Ac methylation arrests H₂O₂-induced cell oxidative stress damage. To explore the possible protective mechanism, we performed iTRAQ-based comparative quantitative proteomics and phosphoproteomics studies of H₂O₂-treated vector control and LCMT1-overexpressing cells.

Results

A total of 4480 non-redundant proteins and 3801 unique phosphopeptides were identified by this means. By comparing the H₂

SUBMITTER: Wang X 

PROVIDER: S-EPMC6748586 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature