Rapid global characterization of immunoglobulin G1 following oxidative stress.

ABSTRACT: Although peroxide and leachable metal-induced chemical modifications are among the most important quality attributes in bioprocess development, there is no mainstream characterization method covering all common modifications theoretically possible on therapeutic proteins that also gives consistent results quickly. Here, we describe a method for rapid and consistent global characterization of leachable metals- or peroxide-stressed immunoglobulin (Ig) G1 monoclonal antibodies (mAbs). Using two independent protease digestions, data-independent acquisition and data-dependent acquisition liquid chromatography high-resolution mass spectrometry, we monitored 55 potential chemical modifications on trastuzumab, a humanized IgG1 mAb. Processing templates including all observed peptides were develope

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6748588 | biostudies-literature | 2019 Aug/Sep

REPOSITORIES: biostudies-literature