Measuring the effects of macromolecular crowding on antibody function with biolayer interferometry.

ABSTRACT: Biotherapeutic proteins are commonly dosed at high concentrations into the blood, which is an inherently complex, crowded solution with substantial protein content. The effects of macromolecular crowding may lead to an appreciable level of non-specific hetero-association in this physiological environment. Therefore, developing a method to characterize the diverse consequences of non-specific interactions between proteins under such non-ideal, crowded conditions, which deviate substantially from those commonly employed for in vitro characterization, is vital to achieving a more complete picture of antibody function in a biological context. In this study, we investigated non-specific interactions between human serum albumin (HSA) and two monoclonal antibodies (mAbs) by static light sc

SUBMITTER: Kim DM 

PROVIDER: S-EPMC6748605 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature