Multispectral tracing in densely labeled mouse brain with nTracer.

ABSTRACT:

Summary

This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.

Availability and implementation

nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial.

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Roossien DH 

PROVIDER: S-EPMC6748755 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature