Microfluidic and computational study of structural properties and resistance to flow of blood clots under arterial shear.

ABSTRACT: The ability of a blood clot to modulate blood flow is determined by the clot's resistance, which depends on its structural features. For a flow with arterial shear, we investigated the characteristic patterns relating to clot shape, size, and composition on the one hand, and its viscous resistance, intraclot axial flow velocity, and shear distributions on the other. We used microfluidic technology to measure the kinetics of platelet, thrombin, and fibrin accumulation at a thrombogenic surface coated with collagen and tissue factor (TF), the key clot-formation trigger. We subsequently utilized the obtained data to perform additional calibration and validation of a detailed computational fluid dynamics model of spatial clot growth under flow. We then ran model simulations to gain insights in

SUBMITTER: Mitrophanov AY 

PROVIDER: S-EPMC6748893 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature