Liquid biopsies using pleural effusion-derived exosomal DNA in advanced lung adenocarcinoma.

ABSTRACT:

Background

Recently, plasma-derived exosomal DNA (exoDNA) has been successfully used in clinical genetic testing. However, the clinical utility of pleural effusion-derived exoDNA (PE-exoDNA) was still unknown. This study aimed to assess the feasibility of using PE-exoDNA for genetic testing in patients with advanced lung adenocarcinoma.

Methods

Twenty PE-exoDNA samples and 18 pleural effusion-derived cell-free DNA (PE-cfDNA) samples were obtained from 20 stage IV lung adenocarcinoma patients. Using targeted next-generation sequencing (NGS) of 416 cancer-relevant genes, the genomic alterations between PE-exoDNA and PE-cfDNA were identified and compared.

Results

NGS results showed highly similar mutation profiles between exoDNA and cfDNA, with TP53, EGFR,

SUBMITTER: Song Z 

PROVIDER: S-EPMC6749121 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature