Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions.

ABSTRACT:

Background

The growing field of plant molecular farming relies on expression vectors that allow high yields of recombinant proteins to be produced through transient gene expression. While numerous expression vectors currently exist for this purpose, there are very few examples of systematic efforts to improve upon these. Moreover, the current generation of expression systems makes use of naturally-occurring regulatory elements, typically selected from plant viruses, to maximise yields. This study aims to use rational design to generate synthetic sequences that can rival existing ones.

Results

In this work, we present the rational design of novel synthetic 5' and 3' untranslated regions (UTRs) which can be used in various combinations to modulate accumulation levels of transi

SUBMITTER: Peyret H 

PROVIDER: S-EPMC6749642 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature