Identification and optimization of PrsA in Bacillus subtilis for improved yield of amylase.

ABSTRACT:

Background

PrsA is an extracytoplasmic folding catalyst essential in Bacillus subtilis. Overexpression of the native PrsA from B. subtilis has repeatedly lead to increased amylase yields. Nevertheless, little is known about how the overexpression of heterologous PrsAs can affect amylase secretion.

Results

In this study, the final yield of five extracellular alpha-amylases was increased by heterologous PrsA co-expression up to 2.5 fold. The effect of the overexpression of heterologous PrsAs on alpha-amylase secretion is specific to the co-expressed alpha-amylase. Co-expression of a heterologous PrsA can significantly reduce the secretion stress response. Engineering of the B. licheniformis PrsA lead to a further increase in amylase secretion and reduced secretion stress.

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SUBMITTER: Quesada-Ganuza A 

PROVIDER: S-EPMC6749698 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature