High-throughput sequencing of circRNAs reveals novel insights into mechanisms of nigericin in pancreatic cancer.

ABSTRACT:

Background

Our previous study had proved that nigericin could reduce colorectal cancer cell proliferation in dose- and time-dependent manners by targeting Wnt/β-catenin signaling. To better elucidate its potential anti-cancer mechanism, two pancreatic cancer (PC) cell lines were exposed to increasing concentrations of nigericin for different time periods, and the high-throughput sequencing was performed to explore the circRNA expression profiles after nigericin exposure on pancreatic cancer (PC) cells.

Results

In this study, a total of 183 common differentially expressed circRNAs were identified, and the reliability and validity of the sequencing data were verified by the PCR analysis. According to the parental genes of circRNAs, the GO analysis was performed to predict the

SUBMITTER: Xu Z 

PROVIDER: S-EPMC6749718 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature