Widespread Translational Control of Fibrosis in the Human Heart by RNA-Binding Proteins.

ABSTRACT:

Background

Fibrosis is a common pathology in many cardiac disorders and is driven by the activation of resident fibroblasts. The global posttranscriptional mechanisms underlying fibroblast-to-myofibroblast conversion in the heart have not been explored.

Methods

Genome-wide changes of RNA transcription and translation during human cardiac fibroblast activation were monitored with RNA sequencing and ribosome profiling. We then used RNA-binding protein-based analyses to identify translational regulators of fibrogenic genes. The integration with cardiac ribosome occupancy levels of 30 dilated cardiomyopathy patients demonstrates that these posttranscriptional mechanisms are also active in the diseased fibrotic human heart.

Results

We generated nucleotide-resolution transl

SUBMITTER: Chothani S 

PROVIDER: S-EPMC6749977 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature