Enhanced Sample Handling for Analytical Ultracentrifugation with 3D-Printed Centerpieces.

ABSTRACT: The centerpiece of the sample cell assembly in analytical ultracentrifugation holds the sample solution between windows, sealed against high vacuum, and is shaped such that macromolecular migration in centrifugal fields exceeding 200 000g can proceed undisturbed by walls or convection while concentration profiles are imaged with optical detection systems aligned perpendicular to the plane of rotation. We have recently shown that 3D printing using various materials allows inexpensive and rapid manufacturing of centerpieces. In the present work, we expand this endeavor to examine the accuracy of the measured sedimentation process, as well as short-term durability of the centerpieces. We find that 3D-printed centerpieces can be used many times and can provide data equivalent in quality to com

SUBMITTER: To SC 

PROVIDER: S-EPMC6750210 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature