Deep-sequencing study of HCV G4a resistance-associated substitutions in Egyptian patients failing DAA treatment.

ABSTRACT:

Purpose

 To study resistance-associated substitutions using next-generation sequencing in Egyptian hepatitis C virus-infected patients failing direct-acting antiviral treatment.

Methods

 The current study describes three cases of treatment failure in patients referred to Zagazig Viral Hepatitis Treatment Center (ZVHTC), Sharkia Governorate, Egypt. RAS were identified and characterized using deep sequencing. The first patient had breakthrough while receiving a daclatasvir (DCV)+sofosbuvir (SOF) regimen, patient 2 relapsed after treatment with DCV+SOF+ribavirin (RBV), and patient 3 relapsed after DCV+SOF therapy. A serum sample was collected from each patient at failure and sent to Vall d'Hebron Research Institute at Hospital Universitari Vall d'Hebron in Barcelona (Spain) for

SUBMITTER: Amer F 

PROVIDER: S-EPMC6750843 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature