LITAF (Lipopolysaccharide-Induced Tumor Necrosis Factor) Regulates Cardiac L-Type Calcium Channels by Modulating NEDD (Neural Precursor Cell Expressed Developmentally Downregulated Protein) 4-1 Ubiquitin Ligase.

ABSTRACT:

Background

The turnover of cardiac ion channels underlying action potential duration is regulated by ubiquitination. Genome-wide association studies of QT interval identified several single-nucleotide polymorphisms located in or near genes involved in protein ubiquitination. A genetic variant upstream of LITAF (lipopolysaccharide-induced tumor necrosis factor) gene prompted us to determine its role in modulating cardiac excitation.

Methods

Optical mapping was performed in zebrafish hearts to determine Ca²⁺ transients. Live-cell confocal calcium imaging was performed on adult rabbit cardiomyocytes to determine intracellular Ca²⁺handling. L-type calcium channel (LTCC) current (I_Ca,L) was measured using whole-cell recording. To study the

SUBMITTER: Moshal KS 

PROVIDER: S-EPMC6750970 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature