Comparison of Human Tissue Microarray to Human Pericyte Transcriptome Yields Novel Perivascular Cell Markers.

ABSTRACT: Human perivascular progenitor cells, including pericytes, are well-described multipotent mesenchymal cells giving rise to mesenchymal stem cells in culture. Despite the unique location of pericytes, specific antigens to distinguish human pericytes from other cell types are few. Here, we employed a human tissue microarray (Human Protein Atlas) to identify proteins that are strongly and specifically expressed in a pericytic location within human adipose tissue. Next, these results were cross-referenced with RNA sequencing data from human adipose tissue pericytes, as defined as a fluorescence activated cell sorting (FACS) purified CD146⁺CD34^-CD31^-CD45^- cell population. Results showed that from 105,532 core biopsies of soft tissue, 229 proteins showe

SUBMITTER: Hsu CY 

PROVIDER: S-EPMC6751392 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature