Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris.

ABSTRACT:

Objective

Glucuronoyl esterase (GE) is an emerging enzyme that improves fractionation of lignin-carbohydrate complexes. However, the commercial availability of GE is limited, which hinders the research of GE-based bioprocesses for its industrial application in lignocellulose biorefineries. This study evaluated a workable, cost-effective, and commercially scalable production strategy to improve the ease of GE-based research. This strategy consisted of a constitutive and methanol-free enzyme production step coupled with a two-step filtration process. The aim was to determine if this strategy can yield copious amounts of GE, by secretion into the extracellular medium with an acceptable purity that could allow its direct application. This approach was further validated for cellobiose d

SUBMITTER: Conacher CG 

PROVIDER: S-EPMC6751620 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature