"Water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation.

ABSTRACT: By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH₃(OCH₂CH₂) _n -Im-t-BuOH][Tf₂N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][Tf₂N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized

SUBMITTER: Zhao H 

PROVIDER: S-EPMC6751713 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature