Closed system RT-qPCR as a potential companion diagnostic test for immunotherapy outcome in metastatic melanoma.

ABSTRACT:

Background

In melanoma, there is no companion diagnostic test to predict response to programmed cell death 1 (PD-1) axis immune checkpoint inhibitor (ICI) therapy. In the adjuvant setting, only one in five patients may benefit from ICI, so a biomarker is needed to select those that may or may not benefit. Here, we test a new 4-gene multiplex immunotherapy panel with research use only (RUO) prototype mRNA expression profile on the GeneXpert closed system using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) for association with clinical benefit after treatment with ICI therapy in metastatic melanoma patients.

Methods

Pretreatment formalin-fixed paraffin-embedded (FFPE) tissue sections from melanoma patients treated with anti-PD-1 therapy (pemb

SUBMITTER: Gupta S 

PROVIDER: S-EPMC6751819 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature