Upper versus lower airway microbiome and metagenome in children with cystic fibrosis and their correlation with lung inflammation.

ABSTRACT:

Objective

Airways of children with cystic fibrosis (CF) harbor complex polymicrobial communities which correlates with pulmonary disease progression and use of antibiotics. Throat swabs are widely used in young CF children as a surrogate to detect potentially pathogenic microorganisms in lower airways. However, the relationship between upper and lower airway microbial communities remains poorly understood. This study aims to determine (1) to what extent oropharyngeal microbiome resembles the lung microbiome in CF children and (2) if lung microbiome composition correlates with airway inflammation.

Method

Throat swabs and bronchoalveolar lavage (BAL) were obtained concurrently from 21 CF children and 26 disease controls. Oropharyngeal and lung microbiota were analyzed using 16

SUBMITTER: Kirst ME 

PROVIDER: S-EPMC6752789 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature