Response of Sulfolobus solfataricus Dpo4 polymerase in vitro to a DNA G-quadruplex.

ABSTRACT: Repetitive DNA sequences support the formation of structures that can interrupt replication and repair, leading to breaks and mutagenesis. One particularly stable structure is G-quadruplex (G4) DNA, which is four-stranded and formed from tandemly repetitive guanine bases. When folded within a template, G4 interferes with DNA synthesis. Similar to non-duplex structures, DNA base lesions can also halt an advancing replication fork, but the Y-family polymerases solve this problem by bypassing the damage. In order to better understand how guanine-rich DNA is replicated, we have investigated the activity of the model Y-family polymerase, Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), on guanine-rich templates in vitro. We find that Dpo4 progression on templates containing either a single

SUBMITTER: Berroyer A 

PROVIDER: S-EPMC6753385 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature